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Microscopy & Imaging Lab  Related Links: Page last reviewed 11/28/2005 by webmaster. |
The lab is a recharge unit and charges for instrument time and supplies. Lab staff can consult and advise anyone interested in doing most kinds of microscopy. Lab staff will train and assist users in aspects of microscopy related projects where specialized skill or training is needed that is beyond that of the user. Staff will not do routine chores associated with microscopy projects that should be within the abilities of reasonably experienced workers. The lab has all the basic equipment needed to do most kinds of light microscopy. Light microscopes include a Leitz Diaplan compound microscope equipped for bright field, fluorescence, and DIC, a Wild M5A stereo microscope and several basic compound and stereo microscopes for specimen preparation and general inspection. The lab operates an ISI WB-6 SEM. This microscope is equipped with a 4Pi digital image capture system and an energy dispersive x-ray system. It also has a Robinson BSE detector and an infrared chamber scope. The current recharge rate for this instrument is $30/hour of beam time. Transmission Electron Microscopy The lab operates two transmission electron microscopes, a JEOL 1200EX and a JEOL 1230. The JEOL 1200EX is available for teaching and research to any qualified user. It is equipped with a Gatan Model 792Bioscan digital camera running on a Windows 2000 computer. Digital images are easily captured and transferred eliminating the need for darkroom work. The recharge rate for this instrument is $30/hour of beam time. The JEOL 1230 is equipped with a LaB6 gun, a Gatan Cryotransfer holder, a Gatan 622 TV camera and a Gatan Ultrascan digital camera. This is a state of the art instrument primarily for work requiring the cryostage and high resolution camera. To inquire about the use of this instrument, contact Jon in the labor Professor Melissa Jurica, MCD Biology, ext. 9-4427, jurica@biology.ucsc.edu. The recharge rate for this instrument has not been established. Jon currently assists in the administration of a Zeiss Pascal confocal microscope that is housed in room 208A Baskin. This instrument is the property of a group of ETOX and MCD Biology researchers, but is available to others if needed. Contact Jon in the lab or Professor Karen Ottemann, ETOX, ext. 9-3482, ottemann@ucsc.edu for information about this microscope. The recharge rate for this instrument is $20/hour. The lab has most of the equipment needed for routine specimen preparation for light and electron microscopy. For light microscopy, the lab has rotary microtomes, warming trays, and staining jars available for paraffin sectioning and a JB-4 microtome is available for plastic sectioning. For SEM, a Balzers Union Critical Point Dryerand Polaron E5300 Sputter Coater are available. For TEM, a Sorvall MT-2B and an AO Ultracutultramicrotome are available along with an LKB glass knife maker. Diamond knives are available for trained users. Additionally, the lab has an SBT Model 590+ Tripod Polisher and an SBT Model 900 Grinder Polisher. Other equipment useful for general specimen preparation includes an Ultrascience 102S high purity glass still, a Pella3450 Microwave Tissue Processor, and a Denton DV 502 Vacuum Evaporator. The lab can assist in some forms of digital imaging. Available are a Polaroid Sprint Scan 35, an Agfa Arcus II flatbed scanner and an HP DesignJet 800PS wide format printer. Campus students and researchers may use the lab’s DesignJet 800PS wide format printer to produce posters for scientific meetings. Costs for poster printing are recharged to campus accounts at reasonable rates. To learn more about requirements and options for poster printing, contact Jon Krupp at ext. 9-2477 or jmkrupp@ucsc.edu.
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